Antiparkinsonian and Antioxidant Effects of Hydroalcoholic Extract of Camellia sinensis, Asparagus racemosus, Mucuna pruriens and their Combination

Objectives: Because of environmental risk factors Parkinson’s disease rate doubled in last decades. In upcoming decades rate of Parkinson’s disease is expected to be 12 million people in aging population. Present treatments are having huge side effects and requires more combinations. Hence objective of this study is to provide herbal combination for Parkinson’s disease with reduced side and adverse effects. Methods: The antiparkinsonian activity of HECS, HEAR, HEMP and Mixture was evaluated by using haloperidol induced catalepsy, reserpine induced hypolocomotion, tacrine induced vacuous chewing movements and orofacial brusts. Antioxidant activity was assessed by using DPPH radical and H₂O₂ scavenging assay. The results were analyzed by repeated measure ANOVA followed by Dunnett’s test. Results: Significant reduction (P<0.05) in haloperidolinduced catalepsy was observed in the all groups at the doses of 30 and 100 mg/kg when given orally. Mixture 30 mg/kg showed extremely significant (P<0.001) reduction in duration of catalepsy. Pretreatment with Mixture at 100mg/kg was significantly (P<0.05) reduces Reserpine induced hypolocomotion which is more significant as compared with other treatments. Similarly, HECS 30 mg/kg and mixture was more effective (P<0.05) than remaining extracts in reducing tacrine induced Vacuous chewing movements. In tacrine induced orofacial brust Mixture and HEAR 30 mg/kg shows extremely significant (P<0.001) reduction of orofacial brusts. Similarly Mixture and HECS 100 mg/kg is more effective than other treatment in tacrine induced tongue protrusion. In DPPH scavenging assay, all the extracts exhibited free radical scavenging activity. In DPPH assay the IC₅₀ value of ascorbic acid, HECS, HEAR, HEMP and Mixture (1:1:1) was 14.99, 18.44, 26.51, 23.19 and 20.47 μg/ml respectively. Conclusion: 1:1:1 mixture show extremely significant antioxidant and anti-parkinsonian activity as compare with individual hydroalcoholic extract. Thus the studied combination possess potent antiparkinsonian effect.