International Journal of Pharmaceutical Investigation, 2020, 10, 2, 146-150.
Published: June 2020
Type: Original Article
Department of Chemistry, SNS College of Technology, Coimbatore – 641035, Tamil Nadu, INDIA.
Department of Chemistry, National College, Trichy-620002, Tamil Nadu, INDIA.
Department of Chemistry, Sri Ramakrishna Engineering College, Coimbatore – 641022, Tamil Nadu, INDIA.
Department of Chemistry, St. Joseph’ College, Trichy – 620002, Tamil Nadu, INDIA.
Objectives: To synthesize silver oxide nanoparticles from the medicinal plant Cyathea nilgirensis Holttum and to evaluate the percentage of inhibition of silver oxide nanoparticles against some selective bacterial species (three gram positive and three gram negative) and two fungal species. Anticancer activity was performed for Ag2O nanoparticles using Trypan Blue Assay Method using Daltons lymphoma ascites cells. Methods: Silver oxide nanoparticles were synthesized by chemical precipitation method using the extract of the medicinal plant Cyathea nilgiriensis Holttum and Silver nitrate. Particle size Analyzer (PSA), X-ray Diffraction Analysis (XRD), Fourier Transform Infrared Spectroscopy (FT-IR), Scanning Electron Microscope Analysis (SEM) and Energy Dispersive X-Ray (EDX) studies studies were employed to confirm the size distribution, composition and crystallographic structure of the Silver oxide nanoparticles. Results: Particle size Analyzer and X-ray diffraction results proved the size and crystalline nature of Ag2O nanoparticles. Fourier transform infrared spectroscopy studies has proved that biomolecules has acted as chapping and stabilizing agents in the synthesis of Ag2O nanoparticle, Scanning electron microscope analysis has exhibited morphological appearance of Ag2O nanoparticles and Energy Dispersive X-Ray analysis has proved the presence of both silver and oxygen in the synthesized Ag2O nanoparticles. On comparing the zone of inhibition of three-gram positive bacterial species, M. luteus (20 mm) has shown larger zone of inhibition (20 mm) and when comparing the zone of inhibition of three-gram negative bacterial species, K. pneumoniae has shown larger zone of inhibition (21 mm). The increase of concentration from 10 μg to 200 μg of Ag2O nanoparticles increases the anticancer efficiency of the biosynthesized Ag2O nanoparticles. Conclusion: The Instrumentation results have proved the characteristic nature of the synthesized Ag2O nanoparticles. Now it’s clear that greener synthesized nanoparticles has proved to be better antimicrobial and anticancer agents.
Keywords: Cyathea nilgiriensis Holttum, Ag2O nanoparticles, Green chemistry, Characterization, antimicrobial and anticancer activity.