International Journal of Pharmaceutical Investigation, 2019, 9, 4, 200-204.
DOI: 10.5530/ijpi.2019.4.37
Published: December 2019
Type: Original Article
Authors:
Shiva Roshankhah
Department of Anatomical Sciences, Medical School, Kermanshah University of Medical Sciences, Daneshgah Ave., Taghbostan, Kermanshah, IRAN.
Amir Abdolmaleki
Department of Anatomical Sciences, Medical School, Kermanshah University of Medical Sciences, Daneshgah Ave., Taghbostan, Kermanshah, IRAN.
Cyrus Jalili
Medical Biology Research Center, Department of Anatomical Sciences, Kermanshah University of Medical Sciences, Daneshgah Ave., Taghbostan, Kermanshah, IRAN.
Mohammad Reza Salahshoor
Department of Anatomical Sciences, Medical School, Kermanshah University of Medical Sciences, Daneshgah Ave., Taghbostan, Kermanshah, IRAN.
ABSTRACT
Objectives: The dentate gyrus (DG) is a part of a brain region known as hippocampus. Falcaria vulgaris (FV) is a vegetable that contains antioxidant ingredients. Nicotine is one of the main risk factors in functional disorders of various organ systems. This study was designed to evaluate the therapeutic effects of FV against nicotine induced injury on the hippocampus DG region of rats. Methods: In this study, 64 male rats were randomly assigned to 8 groups: Sham group, nicotine group (0.5 mg/kg), FV groups (50, 100 and 150 mg/kg) and nicotine + FV groups. Treatments were administered intraperitoneally daily for 28 days. Ferric Reducing Antioxidant Power (FRAP) method was applied to determine the total antioxidant capacity. The number of dendritic spines was investigated by Golgi technique. Cresyl violet method was used to determine the number of neurons in DG region. Also, Griess technique was used to determine serum nitrite oxide level. Results: Nicotine administration increased significantly nitrite oxide level and total antioxidant capacity and a decreased number of neuronal dendritic spines and neurons compared to the sham group (P < 0.05). In the FV and nicotine + FV groups, in all dosages, the number of neurons and neuronal dendritic spines increased significantly while nitrite oxide level and total antioxidant capacity decreased compared to the nicotine group (P < 0.05). Conclusion: It seems that FV administration improves DG region injury in rates because of nicotine.
Keywords: Falcaria vulgaris, Dentate gyrus, Nicotine, Hippocampus, Rats.