International Journal of Pharmaceutical Investigation, 2019, 9, 1, 2-6.
DOI: 10.5530/ijpi.2019.1.2
Published: March 2019
Type: Original Article
Authors:
Roshankhah Shiva
Department of Anatomical Sciences, Medical School, Kermanshah University of Medical Sciences, Kermanshah, IRAN.
Arash Ziapoor
Health Education and Health Promotion, Kermanshah University of Medical Sciences, Kermanshah, IRAN.
Cyrus Jalili
Medical Biology Research Center, Kermanshah University of Medical Sciences, Kermanshah, IRAN.
Mohammad Reza Salahshoor
Department of Anatomical Sciences, Medical School, Kermanshah University of Medical Sciences, Kermanshah, IRAN.
ABSTRACT
Background: Proteins play the most important role in the structure and function of liver. Royal Jelly (RJ) as a honey bee secretion has antioxidant activities. This study was designed to evaluate the effects of RJ against hepatic damage in rats induced by Low Protein Diet (LPD). Materials and Methods: Forty‑eight male rats were randomly assigned into 6 groups: sham and LPD (%8 protein) groups; RJ groups (200 mg/kg RJ for 5 months and 200 mg/kg RJ for 10 months, orally) and LPD + RJ groups (200 mg/kg RJ orally + LPD for 5 months and 200 mg/kg RJ orally and LPD for 10 months). Griess technique was hired for determination of serum Nitric Oxide (NO) level. Aspartate Aminotransferase (AST), Alanine Aminotransferase (ALT) and Alkaline Phosphatase (ALP) concentrations were determined for liver functional disturbances value. In addition, Thiobarbituric acid reactive species, antioxidant capacity, the diameter of hepatocytes and the Central Hepatic Vein (CHV) were investigated. Results: LPD significantly increased the liver Malondialdehyde (MDA) and NO levels, the mean diameter of CHV and hepatocyte, hepatic enzymes and decreased tissue Ferric Reducing Ability of Plasma (FRAP) level compared to the sham group (P< 0.01). The RJ and RJ + LPD in all treatments significantly reduced the mean diameter of hepatocyte and CHV, hepatic enzymes, liver MDA and NO levels and increased tissue FRAP level compared to the LPD group (P< 0.01). Conclusion: RJ administration recovers the hepatic injury due to oxidative stress by LPD in rats.
Keywords: Low protein diet, Liver, Hepatoprotective, Royal jelly, Rats.