International Journal of Pharmaceutical Investigation, 2018, 8, 3, 130-137.
DOI: 10.4103/jphi.JPHI_25_18
Published: July 2018
Type: Original Article
Authors:
Suhasini Donthi
Department of Biotechnology, Sreenidhi Institute of Science and Technology, India.
Jayasree Ganugapati
Department of Biotechnology, Sreenidhi Institute of Science and Technology, India.
Vijayalakshmi Valluri
Immunology Unit, Bhagwan Mahavir Hospital and Research Center, India.
Ramesh Macha
Department of Chemistry, Osmania University, Hyderabad, Telangana, India.
Krovvidi S. R. Sivasai
Department of Biotechnology, Sreenidhi Institute of Science and Technology, India.
ABSTRACT
Background: Arginine being a common substrate for arginase and nitric oxide synthase (NOS) an imbalance between enzymes could lead to a shift in airway responses. Reports suggest that increased arginase reduces the substrate availability to NOS and attributes to the airway hyperresponsiveness. Hence, inhibition of arginase might enhance the bioavailability of arginine to NOS and generates nitric oxide (NO) a bronchodilator. Molecules from Ephedra and Eugenia caryophyllus are documented for bronchodilator properties. However, the mechanism of action of these molecules for enhancing bronchodilation is not well characterized. The objective of the present study is to assess whether these molecules could inhibit the arginase by binding at its active site and helps in bronchodilation using in silico approach. Methods: The crystal structures of the arginase and NOS enzymes were selected from the protein database. The molecules from Ephedra and Eugenia caryophyllus were obtained from Pubchem. Drug likeliness and bioactivity of the molecules were assessed by Molinspiration. The successful molecules were docked with active sites of enzymes using docking software, and the docked complexes were analyzed using Accelrys Discovery Studio. Results: Molecules from Ephedra and Eugenia caryophyllus were able to interact to arginase at the active site whereas away from the active site in case of NOS. The molecules showed differential binding affinities, and some of them had higher binding affinity than substrate arginine. Conclusion: In silico study suggests that molecules of Ephedra and Eugenia were capable of blocking the active site of arginase. We speculate that if these molecules are used as therapeutics, they could inhibit the arginase activity and this might increase arginine availability to NOS to produce NO which acts as bronchodilator. Our study suggests that molecules which bind to active site of arginine and do not affect the active site of NOS might be the potential molecules for arginase associated asthma.
Keywords: ArgusLab, Cambridge Crystallographic Data Center Genetic Optimization for Ligand Docking, Discovery studio, Genetic optimization, Virtual screening.