International Journal of Pharmaceutical Investigation, 2019, 9, 3, 128-134.
DOI: 10.5530/ijpi.2019.3.24
Published: November 2019
Type: Original Article
Authors:
Achintya Kumar Mandal
Siddha Central Research Institute, (Central Council for Research in Siddha, Ministry of AYUSH, Government of India), Anna Hospital Campus, Arumbakkam, Chennai, Tamil Nadu, INDIA
Ramachandran Shakila
Siddha Central Research Institute, (Central Council for Research in Siddha, Ministry of AYUSH, Government of India), Anna Hospital Campus, Arumbakkam, Chennai, Tamil Nadu, INDIA
Sujith Thatipelli
Siddha Central Research Institute, (Central Council for Research in Siddha, Ministry of AYUSH, Government of India), Anna Hospital Campus, Arumbakkam, Chennai, Tamil Nadu, INDIA
Mattummal Rubeena
Siddha Central Research Institute, (Central Council for Research in Siddha, Ministry of AYUSH, Government of India), Anna Hospital Campus, Arumbakkam, Chennai, Tamil Nadu, INDIA
Kopala Narayanan Sunilkumar
Siddha Central Research Institute, (Central Council for Research in Siddha, Ministry of AYUSH, Government of India), Anna Hospital Campus, Arumbakkam, Chennai, Tamil Nadu, INDIA
Arumugam Thilagam
Siddha Central Research Institute, (Central Council for Research in Siddha, Ministry of AYUSH, Government of India), Anna Hospital Campus, Arumbakkam, Chennai, Tamil Nadu, INDIA
Rethinam Ganesan
Siddha Central Research Institute, (Central Council for Research in Siddha, Ministry of AYUSH, Government of India), Anna Hospital Campus, Arumbakkam, Chennai, Tamil Nadu, INDIA
ABSTRACT
Objectives: To evaluate quality standards and to screen antioxidant activity of Cuvacakutori Chooranam (CC) a poly herbomineral Siddha formulation. Methods: The drug was prepared with authenticated ingredients. Powder microscopic, phytochemical, physicochemical, HPTLC fingerprint profiling (n-hexane, successive chloroform, successive ethanol), total phenol and flavonoid estimation, 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging and superoxide scavenging activities of CC were studied. Results: Microscopic studies revealed diagnostic features to identify the formulation using microscope. Preliminary phytochemical screening revealed the presence of different secondary metabolites, unique chromatographic fingerprint and antioxidant activities. Conclusion: The powder microscopic characters and the evolved physicochemical data arrived could serve as a reference tool for quality control. The presence of different categories of phytochemicals and antioxidant activity of the drug gives broad scope for further efficacy studies of this drug.
Keywords: Respiratory diseases, Cuvacakutori chooranam, Standardization, HPTLC, Antioxidant.