International Journal of Pharmaceutical Investigation, 2012, 2, 2, 78-82.
DOI: 10.4103/2230-973X.100042
Published: August 2012
Type: Original Article
Authors:
Sonjoy Mandal
Department of Industrial Pharmacy, Bapuji Pharmacy College, Davangere-577004, Karnataka, India.
Manjunath KMJ Thimmasetty
Department of Industrial Pharmacy, Bapuji Pharmacy College, Davangere-577004, Karnataka, India.
GL Prabhushankar
Department of Industrial Pharmacy, Bapuji Pharmacy College, Davangere-577004, Karnataka, India.
Geetha MS
Department of Industrial Pharmacy, Bapuji Pharmacy College, Davangere-577004, Karnataka, India.
ABSTRACT
Background/Aim: The aim of the present investigation is to prepare and evaluate in situ gel-forming ophthalmic drug delivery system of moxifloxacin hydrochloride. Materials and Methods: Sodium alginate, a novel ophthalmic gel-forming mucoadhesive polymer, which gets converted to gel in the presence of divalent-cations (calcium ion) present in the lachrymal fluid, was used as the gelling agent. Hydroxy propyl methyl cellulose (HPMC) is a mucoadhesive polymer used as viscosity enhancer. Suitable concentrations of buffering agents were used to adjust the pH to 6.5. All the formulations were sterilized in an autoclave at 121°C for 15 minutes. The formulations were evaluated for clarity, pH measurement, gelling capacity, drug content estimation, rheological study, in vitro diffusion study, antibacterial activity, isotonicity, and eye irritation study. Results: The developed formulations exhibited sustained release of drug from formulation over a period of 10 hours thus increasing residence time of the drug. The optimized formulations were tested for eye irritation on albino rabbit (male). The formulations were found to be non-irritating with no ocular damage or abnormal clinical signs to the cornea, iris or conjunctiva observed. Conclusion: These in situ gelling systems containing gums may be a valuable alternative to the conventional systems..
Keywords: Gelling Capacity, In situ gel, In vitro, Diffusion Study, Moxifloxacin Hydrochloride, Rheological Evaluation