International Journal of Pharmaceutical Investigation, 2022, 12, 4, 430-437.
DOI:10.5530/ijpi.2022.4.74
Published: October 2022
Type: Original Article
Authors:
Rameshor Singh Atom
[1]Department of Chemistry, Waikhom Mani Girls’ College, Thoubal, Manipur, INDIA.[2]Department of Chemistry, Manipur University, Imphal, Manipur, INDIA.
Shaukat Ali M Shaikh
[1]School of Chemical Sciences, Centre for Excellence in Basic Sciences, Mumbai University, Mumbai, Maharashtra, INDIA.[2]Department of Atomic Energy, Mumbai University, Mumbai, Maharashtra, INDIA.
Kishorjit Maharabam
Department of Chemistry, Manipur University, Imphal, Manipur, INDIA.
Rakhi Khunjamayum
Department of Biochemistry, Manipur University, Imphal, Manipur, INDIA.
Dini Ahanthem
Department of Chemistry, Manipur University, Imphal, Manipur, INDIA.
Warjeet Singh Laitonjam
Department of Chemistry, Manipur University, Imphal, Manipur, INDIA.
Raghumani Singh Ningthoujam
Chemistry Division, Bhabha Atomic Research Centre, Mumbai, Maharashtra, INDIA.
Amit Kunwar
Radiation and Photochemistry Division, Bhabha Atomic Research Centre, Mumbai, Maharashtra, INDIA.
ABSTRACT
Bakground: Kaempferia parviflora (zingiberaceae family) rhizomes are used for various pharmacological applications. The present study is aimed for putative identification of phyto-constituents in the chloroform extract of the rhizome of Kaempferia parviflora and to correlate with bioactivities like antioxidant and anti-microbial/fungal activities. Methods: Rhizome extract was prepared using Soxhlet extractor and undergone the phytoconstituents analysis of it by gas chromatography–mass spectrometry (GC-MS). Results: Identified twenty-five compounds, out of which 4H-1-Benzopyran- 4-0ne,5,7-dimethoxy-2-phenyl and Hydroxyurea showed the maximum and minimum relative abundance of 76.4% and 0.002% respectively. By agar well diffusion method, the extract (25 μg/ml) exhibited highest antibacterial potential against Micrococcus luteus (MTCC 106) (gram-positive bacteria) and anti-fungal potential against plant pathogen Rhizoctonia solani (MTCC 4633). The extract also showed the potent scavenging activities of 2,2-diphenyl-1-picryl-hydrazine (DPPH) and 2,2′-Azino-bis (3-Ethylbenzothiazoline-6-Sulfonic Acid) (ABTS) radicals with IC50 values of 3.0 ± 0.1 mg/ml and 19.4 ± 1.2 μg/ml respectively. Subsequently, lipid peroxidation assay was also used for estimation of antioxidant activity of this extract. The extract (20 μg/ml) inhibited lipid peroxidation by 80.0 ± 4.5%. Conclusion: The outcomes of present study provide scope for isolation of bioactive compounds from Kaempferia parviflora for medicinal application.
Keywords: GC-MS, Kaempferiaparviflora, Phytochemicals, Rhizome, Bioactivity