International Journal of Pharmaceutical Investigation, 2022, 12, 3, 340-345.
DOI: 10.5530/ijpi.2022.3.57
Published: July 2022
Type: Original Article
Authors:
Shamli S Gupte
UNESCO Trace Element Satellite Centre, School of Studies in Zoology, Jiwaji University, Gwalior, Madhya Pradesh, INDIA.
Arti Rathour
UNESCO Trace Element Satellite Centre, School of Studies in Zoology, Jiwaji University, Gwalior, Madhya Pradesh, INDIA.
Divya Gupta
UNESCO Trace Element Satellite Centre, School of Studies in Zoology, Jiwaji University, Gwalior, Madhya Pradesh, INDIA.
Richa Soni
UNESCO Trace Element Satellite Centre, School of Studies in Zoology, Jiwaji University, Gwalior, Madhya Pradesh, INDIA.
Sadhana Shrivastava
UNESCO Trace Element Satellite Centre, School of Studies in Zoology, Jiwaji University, Gwalior, Madhya Pradesh, INDIA.
Monika Bhaduria
Department of Zoology, Guru Ghasidas Vishwavidyalaya, Bilaspur, Chhattisgarh, INDIA.
Satendra Kumar Nirala
Department of Rural Technology and Social Development, Guru Ghasidas Vishwavidyalaya, Bilaspur, Chhattisgarh, INDIA
Shubham Singh
Department of Zoology, Guru Ghasidas Vishwavidyalaya, Bilaspur, Chhattisgarh, INDIA.
Anjali Sharma
UNESCO Trace Element Satellite Centre, School of Studies in Zoology, Jiwaji University, Gwalior, Madhya Pradesh, INDIA
Deepa Yadav
UNESCO Trace Element Satellite Centre, School of Studies in Zoology, Jiwaji University, Gwalior, Madhya Pradesh, INDIA
Samrat Rakshit
Department of Zoology, Guru Ghasidas Vishwavidyalaya, Bilaspur, Chhattisgarh, INDIA
Sangeeta Shukla
UNESCO Trace Element Satellite Centre, School of Studies in Zoology, Jiwaji University, Gwalior, Madhya Pradesh, INDIA.
ABSTRACT
Background: Tephrosia purpurea (TP), commonly known as wild indigo, is traditionally used in treatment of splenomegaly. It is an important ingredient of various Ayurvedic medicines used in treatment of liver diseases. Objectives: Thus, the study was undertaken to investigate the hepatoprotective efficacy of ethanolic extract of TP against Aflatoxin B1 (AFB1) induced liver injury. Materials and Methods: The ethanolic extract of TP was prepared by Soxhlet extraction method. Presence of polyphenols and antioxidant potential were assessed. The antiproliferative activity of extract was tested on HepG2 cells using MTT assay. For in vivo studies female Wistar rats were randomly divided into 6 groups with 6 animals in each. The entire regime was of 33 days. AFB1 was administered at 200 μg/kg dose and TP was administered at three different doses (100, 200 and 300 mg/kg). 24 hr after last treatment the animals were euthanised and liver and blood samples were collected. Results: 45.77± 2.53 μg/ ml IC50 of extract was seen on HepG2 cells. A significant elevation in serum transaminases, triglycerides (TG) and LPO was seen after AFB1 intoxication. Whereas decline in activities of GSH, SOD, CAT, G6Pase and ATPase were observed. Treatment with different doses of TP restored its activities towards normal indices. Maximum recovery was seen with 300 mg/kg dose of TP. Conclusion: It may be concluded that TP possess hepatoprotective efficacy against AFB1 induced oxidative injury and it may prove to be of clinical use after further studies
Keywords: Aflatoxin B1, Oxidative injury, Liver, Tephrosia purpurea, Antioxidant status.