International Journal of Pharmaceutical Investigation, 2021, 11, 4, 354-357.
DOI: 10.5530/ijpi.2021.4.63
Published: December 2021
Type: Original Article
Authors:
Suresh Babu Bastipati
Centre for Biotechnology, Institute of Science and Technology, Jawaharlal Nehru Technological University, Hyderabad, Telangana, INDIA.
Chepuri Kalyani
Centre for Biotechnology, Institute of Science and Technology, Jawaharlal Nehru Technological University, Hyderabad, Telangana, INDIA.
Chidepudi Devi Sri Lalitha Naga Tulasi
Centre for Biotechnology, Institute of Science and Technology, Jawaharlal Nehru Technological University, Hyderabad, Telangana, INDIA.
Lavudi Saida
Centre for Biotechnology, Institute of Science and Technology, Jawaharlal Nehru Technological University, Hyderabad, Telangana, INDIA.
ABSTRACT
Objectives: In the present study, the anticancer potential of the plant extracts of Elytraria acaulis L. (E. acaulis L.) were investigated on human breast cancer cells (MDAMB-231). Methods: Different organic solvent extras.cts of E. acaulis L. were prepared using methanol, ethanol, ethyl acetate and hexane. The cytotoxicity of plant extracts were evaluated by MTT assay (-(4, 5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide) against MDA MB-231cells. The apoptosis associated morphological changes were also observed with the help of propidium iodide staining method. Results: Of all the solvent extracts, methanol extract has shown the highest activity and its IC50 concentration was found to be 80.8±2.08 μg/ml. The anticancer drug Cis-diammineplatinum (II) dichloride was considered as a standard. Conclusion: In further the bioactive compound should be isolated from the methanol crude extract, which is responsible for the cytotoxic effect
Keywords: E. acaulis L, Anticancer activity, Cytotoxic assay, Propidium iodide, Apoptosis.