International Journal of Pharmaceutical Investigation, 2021, 11, 1, 46-51.
DOI: 10.5530/ijpi.2021.1.9
Published: April 2021
Type: Original Article
Authors:
Shaileyee Das
Pharmacognosy and Phytotherapy Research Laboratory, Division of Pharmacognosy, Department of Pharmaceutical Technology, Jadavpur University, Kolkata, West Bengal, INDIA.
Subhash C Mandal
Pharmacognosy and Phytotherapy Research Laboratory, Division of Pharmacognosy, Department of Pharmaceutical Technology, Jadavpur University, Kolkata, West Bengal, INDIA.
ABSTRACT
Objectives: Zingiber officinale Linn. is a rhizome commonly consumed as a culinary purpose, medicine or spice in India. The aim of this study was to evaluate potent anticancer leads from ginger rhizome peel. Methods: Cytotoxicity study was carried out by using brine shrimp, Antimitotic study was carried out through onion root method, Anti-proliferating study was carried out using in vitro cell line study and at last the cell viability study was carried out through yeast model using quantitative and qualitative methods. Results: LC50 of phenolic group contain fraction (PHGF) of Petroleum ether fraction (PEF) was found to be 18 μg/ml, which was threefold higher than that of synthetic standard podophyllotoxin, i.e., 50 μg/ml. 2 isolated compounds (6-shogaol and 6-gingerol) have good HSF1 inhibition activity and creating apoptosis. 6-shogaol showed good Green fluorescent protein (GFP) at 10μg/ml and 6-gingerol compound good GFP at only 5 μg/ml. In MTT assay cell viability was showing ±54% in ginger peel extract sample dose of 100μg/ml. When the pet ether fraction of ginger peel extract was tested, result showed the ±96 % cell death in dose of 100μg/ml. We observed cell viability of c.90%, 60% and 94% in the case of the wild-type strain. Conclusion: This study revealed the strong antiproliferative potentials of Petroleum ether fraction (PEF) of extract of Ginger peel. It was also found to be moderately safe for consumption and thus could serve as a source of candidate for the development of new antiproliferative and antimicrobial drugs.
Keywords: Anticancer, Antiproliferative, Anti-mitotic, Yeast model, Zingiber officinale Linn.