International Journal of Pharmaceutical Investigation, 2021, 11, 1, 99-103.
Published: April 2021
Type: Original Article
Ashish Kumar Verma
Department of Pharmacology, Teerthanker Mahaveerr College of Pharmacy, TMU, Moradabad, Uttar Pradesh, INDIA
Mamta Farswan Singh
Department of Pharmacology, Sardar Bhagwan Singh University, Dehradun, Uttarakhand, INDIA
Department of Pharmacology, Vivek Ccollege of Technical Education, Bijnor, Uttar Pradesh, INDIA
Objectives: To evaluate the effect of MAP kinase inhibitor (SB239063) on neuroinflammation along with Vitamin B12. Materials and Methods: Male wistar albino rats divided in to eight groups each group having six animals. Neuroinflammation was induced in animals by the lipopolysaccharides/ LPS (100μg/ml) fallowed by treatment with MAPK inhibitor (SB239063) alone (5mg/kg) and along with Vit B12 (0.5mg/kg) for 30 days. Subsequently, animals were screened in the behavioral tests by using radial arm apparatus and actophotometer apparatus followed by biochemical and histopathological estimations. Results: Chronic LPS, 100 μg/kg i.p., caused physical and mental abnormalities in animals, including memory impairment, reduced locometer activity abnormal posture and cognitive deficit. Biochemical analysis of brain homogenate in LPS treated rats showed altered biochemical marker. Neurohistological alterations of various parts of brain of LPS treated rats exhibit severe neuronal space, irregular damaged cells. MAP Kinase inhibitor (SB239063) alone and along with Vit B12 administered for 15 days significantly improved motor performance and cognitive behavior task and restored the histopathological changes. Conclusion: MAP Kinase inhibitor (SB239063) alone show significant effect at dose 5 mg/kg, I.V and whenever it was used in combination at reduced concentration with Vitamin B12 provided slow neuroprotective effect.
Keywords: p38 MAP Kinase, Neuroinflammation, Vitamin B12, Neurodegenerative diseases, Lipopolysaccharide.