Development and Characterization of Gelatin Based Nanoparticles for Targeted Delivery of Zidovudine

  • Namdeo R Jadhav Department of Pharmaceutics, Bharati Vidyapeeth College of Pharmacy, Kolhapur, Maharashtra, India
  • Jadhav S Tone Department of Pharmaceutics, Bharati Vidyapeeth College of Pharmacy, Kolhapur, Maharashtra, India
  • Preeti V Irny Department of Pharmaceutics, Bharati Vidyapeeth College of Pharmacy, Kolhapur, Maharashtra, India
  • Sameer J Nadaf Department of Pharmaceutics, Bharati Vidyapeeth College of Pharmacy, Kolhapur, Maharashtra, India
Keywords: Desolvation, Mannosylation, Particle size, Release, Reticuloendothelial system uptake, Zidovudine nanoparticles

Abstract

Introduction: The present work was aimed at development and evaluation of zidovudin (AZT) loaded gelatin nanoparticles (GNPs) by simple desolvation method and further couple it with mannose. Material and Methods: Total seven batches of GNPs (A1-A7) were formulated by changing the concentration of polymer gelatin. Various parameters such as particle size, polydispersity index, zeta potential, % entrapment efficiency and in- vitro drug release of plain and mannosylated gelatin nanoparticles (M-GNPs) were studied. Results: Scanning electron microscopy (SEM) studies revealed that the average particle size of GNPs and M-GNPs were found to be 394 ± 3.21 and 797.2 ± 2.89 nm respectively (optimised batch A3). It was interesting to note that the average particle size of M-GNPs was more due to anchored mannose, whereas drug entrapment was lesser compared to plain GNPs. Studies have showed drug loading for GNPs and M-GNPs to be 66.56% and 58.85% respectively. Zeta potential studies demonstrated little reduction in solution stability of M-GNPs compared to GNPs. In- vitro drug release studies showed almost 80% release (bimodal) up to 24 h, following Korsmeyer-Peppas release kinetics model (GNPs, r = 0.9760; M-GNPs, r = 0.9712). Conclusions: Hence, it can be concluded that, development of GNPs and M-GNPs will pave the way for reticuloendothelial system uptake of AZT; thus, achieving targeted delivery, selectivity and reduction in associated side effect reduction in acquired immuno defficiency syndrome.

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Scanning electron microscopy microphotographs of drug loaded (a) gelatin nanoparticles (magnification × 82.53 K) (b) mannosylated gelatin nanoparticles (magnification × 15.22 K)
Published
2013-10-03
How to Cite
1.
Jadhav NR, Tone JS, Irny PV, Nadaf SJ. Development and Characterization of Gelatin Based Nanoparticles for Targeted Delivery of Zidovudine. ijpi [Internet]. 3Oct.2013 [cited 4Oct.2022];3(3):126-30. Available from: https://jpionline.org/index.php/ijpi/article/view/86