Polymeric Nanocarrier system Bearing Anticancer Agent for the Treatment of Prostate Cancer: Systematic Development and in vitro Characterization

  • Pritish Kumar Panda Department of Pharmaceutical Sciences, Dr. Harisingh Gour University, Sagar, Madhya Pradesh, INDIA.
  • Sanjay K Jain Department of Pharmaceutical Sciences, Dr. Harisingh Gour University, Sagar, Madhya Pradesh, INDIA. https://orcid.org/0000-0002-9241-3114
Keywords: Polymeric nanoparticles, Targeting, Doxorubicin, PLGA, Prostate cancer.

Abstract

Objectives: Doxorubicin-bearing polymeric (PLGA) nanoparticles (PNPs) were prepared for the treatment of prostate cancer. Materials and Methods: These PNPs were prepared using the solvent evaporation method and characterized using UV, NMR, Particle size Analyser, SEM, and TEM for the determination of shape, size, zeta potential, and polydispersity index. Moreover, in vitro drug release, SRB assay, apoptosis, and haemolytic study were also performed to prove its potentiality for prostate cancer. Results: The mean particle size (MPS), polydispersity index (PDI), and zeta potential (ZP) of PNPs were found to be 101.3 ± 1.23 nm, 0.240 ± 0.28 and -3.11 ± 1.96 mV, respectively. SEM and TEM revealed that the PNPs are spherical in shape and the sizes are approximately 100 nm. The entrapment efficiency, loading efficiency, and percentage drug release of doxorubicin from PNPs were found to be 69.38 ± 1.76%, 4.2 ± 0.64% and 77.56 ± 4.24%, respectively. In addition, cellular apoptosis against PC-3 cell lines was found to be ≥ 5.15 fold and haemolysis toxicity was reduced to ≤ 3.5 fold with PNPs as compared to free drug. Conclusion: These findings demonstrated that PNPs have the potential to deliver the anticancer agent to tumor sites and could be an emerging strategy for the treatment of prostate cancer.

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(a) and (b) Mean Particle Size (MPS), PDI and ZP images (c), (d), and (e) NMR Spectroscopy, Scanning Electron Microscope (SEM) and Transmission Electron Microscope (TEM) images of PNPs.
Published
2022-12-16
How to Cite
1.
Panda PK, Jain SK. Polymeric Nanocarrier system Bearing Anticancer Agent for the Treatment of Prostate Cancer: Systematic Development and in vitro Characterization. ijpi [Internet]. 16Dec.2022 [cited 27Jan.2023];13(1):87-3. Available from: https://jpionline.org/index.php/ijpi/article/view/1799