GC-MS Profiling, in vitro Antioxidant and Antimicrobial Activities of Kaempferia parviflora Wall. ex. Baker Rhizome Extract

  • Rameshor Singh Atom [1]Department of Chemistry, Waikhom Mani Girls’ College, Thoubal, Manipur, INDIA.[2]Department of Chemistry, Manipur University, Imphal, Manipur, INDIA.
  • Shaukat Ali M Shaikh [1]School of Chemical Sciences, Centre for Excellence in Basic Sciences, Mumbai University, Mumbai, Maharashtra, INDIA.[2]Department of Atomic Energy, Mumbai University, Mumbai, Maharashtra, INDIA.
  • Kishorjit Maharabam Department of Chemistry, Manipur University, Imphal, Manipur, INDIA.
  • Rakhi Khunjamayum Department of Biochemistry, Manipur University, Imphal, Manipur, INDIA.
  • Dini Ahanthem Department of Chemistry, Manipur University, Imphal, Manipur, INDIA.
  • Warjeet Singh Laitonjam Department of Chemistry, Manipur University, Imphal, Manipur, INDIA.
  • Raghumani Singh Ningthoujam Chemistry Division, Bhabha Atomic Research Centre, Mumbai, Maharashtra, INDIA.
  • Amit Kunwar Radiation and Photochemistry Division, Bhabha Atomic Research Centre, Mumbai, Maharashtra, INDIA.
Keywords: GC-MS, Kaempferia parviflora, Phytochemicals, Rhizome, Bioactivity

Abstract

Bakground: Kaempferia parviflora (zingiberaceae family) rhizomes are used for various pharmacological applications. The present study is aimed for putative identification of phyto-constituents in the chloroform extract of the rhizome of Kaempferia parviflora and to correlate with bioactivities like antioxidant and anti-microbial/fungal activities. Methods: Rhizome extract was prepared using Soxhlet extractor and undergone the phytoconstituents analysis of it by gas chromatography–mass spectrometry (GC-MS). Results: Identified twenty-five compounds, out of which 4H-1-Benzopyran- 4-0ne,5,7-dimethoxy-2-phenyl and Hydroxyurea showed the maximum and minimum relative abundance of 76.4% and 0.002% respectively. By agar well diffusion method, the extract (25 μg/ml) exhibited highest antibacterial potential against Micrococcus luteus (MTCC 106) (gram-positive bacteria) and anti-fungal potential against plant pathogen Rhizoctonia solani (MTCC 4633). The extract also showed the potent scavenging activities of 2,2-diphenyl-1-picryl-hydrazine (DPPH) and 2,2′-Azino-bis (3-Ethylbenzothiazoline-6-Sulfonic Acid) (ABTS) radicals with IC50 values of 3.0 ± 0.1 mg/ml and 19.4 ± 1.2 μg/ml respectively. Subsequently, lipid peroxidation assay was also used for estimation of antioxidant activity of this extract. The extract (20 μg/ml) inhibited lipid peroxidation by 80.0 ± 4.5%. Conclusion: The outcomes of present study provide scope for isolation of bioactive compounds from Kaempferia parviflora for medicinal application.

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Agar plate shows antifungal activity of BR-C (chloroform extract) in terms of the zone of inhibition (mm) against plant pathogen (R. solani).
Published
2022-10-06
How to Cite
1.
Atom RS, Shaikh SAM, Maharabam K, Khunjamayum R, Ahanthem D, Laitonjam WS, Ningthoujam RS, Kunwar A. GC-MS Profiling, in vitro Antioxidant and Antimicrobial Activities of Kaempferia parviflora Wall. ex. Baker Rhizome Extract. ijpi [Internet]. 6Oct.2022 [cited 6Dec.2022];12(4):430-7. Available from: https://jpionline.org/index.php/ijpi/article/view/1572