Cloning and Expression of Helicobacter pylori ulcer Associated Gene - iceA1 in Brinjal (Solanum melongena L.)

  • Mohammad Javad Mehran JSS Research Foundation, SJCE Technical Campus, Mysore, Karnataka, INDIA.
  • Basaralu Yadurappa Sathish Kumar [1] JSS Research Foundation, SJCE Technical Campus, Mysore, Karnataka, INDIA.[2] Department of Biotechnology, JSS College, Ooty Road, Mysore, Karnataka, INDIA.
  • Nanjundappa Haraprasad JSS Science and Technology University, SJCE, Technical Campus, Mysore, Karnataka, INDIA.
  • Rambod Barzigar JSS Research Foundation, SJCE Technical Campus, Mysore, Karnataka, INDIA.
  • Sayan Paul [5] Department of Biotechnology, Manonmaniam Sundaranar University, Tirunelveli, Tamilnadu, INDIA. [6] Centre for Cardiovascular Biology and Disease, Institute for Stem Cell Science and Regenerative Medicine (inStem), Bangalore, INDIA.
Keywords: Helicobacter pylori, Transgenic brinjal, iceA1, Agrobacterium, Edible Vaccine

Abstract

Background: Plant biotechnology revolutionized the branch of plant sciences contributing to improving crop production. Helicobacter pylori was predominantly seen among all the sectors of the human population, closely stuck to stomach ulcers and cancer. Even though traditional vaccines are effective in preventing H. pylori, edible and non-edible parts of special transgenic plants were also being generated to express its antigens which could elicit an immune response after consuming. The induce by contact with epithelium (iceA1) protein of H. pylori is associated with virulence and stated as predisposing factor to stomach cancer. Objectives: This study intends to study the expression levels of iceA1 gene in the brinjal plant. Materials and Methods: The present study, magnified the iceA1 gene from H. pylori strain 26695 and transformed it into callus of brinjal (from leaf explants) through Agrobacterium tumefacians (EHA105). pBI121 vector was used to construct the plant expression vector, and the transgenic plants generated were further validated through gene amplification and protein expression studies. Results: Out of the 46 plants obtained from this study, five of them were found to be positive for the iceA1 expression as seen on validation assays like gene amplification and protein expression studies. Conclusion: The study could beneficially produce a vaccine candidate against H. pylori from transgenic brinjal. In Addition, it could provide, valid scientific data which can further be used by researchers.

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Image showing the iceA1 gene amplification separated on 1% agarose gel
Published
2021-10-13
How to Cite
1.
Javad Mehran M, Yadurappa Sathish Kumar B, Haraprasad N, Barzigar R, Paul S. Cloning and Expression of Helicobacter pylori ulcer Associated Gene - iceA1 in Brinjal (Solanum melongena L.). ijpi [Internet]. 13Oct.2021 [cited 9Dec.2021];11(3):338-44. Available from: https://jpionline.org/index.php/ijpi/article/view/1189