Anticancer Activity of Elytraria acaulis L. Extracts on Triple Negative Breast Cancer Cell Line

  • Suresh Babu Bastipati Centre for Biotechnology, Institute of Science and Technology, Jawaharlal Nehru Technological University, Hyderabad, Telangana, INDIA.
  • Chepuri Kalyani Centre for Biotechnology, Institute of Science and Technology, Jawaharlal Nehru Technological University, Hyderabad, Telangana, INDIA.
  • Chidepudi Devi Sri Lalitha Naga Tulasi Centre for Biotechnology, Institute of Science and Technology, Jawaharlal Nehru Technological University, Hyderabad, Telangana, INDIA.
  • Lavudi Saida Centre for Biotechnology, Institute of Science and Technology, Jawaharlal Nehru Technological University, Hyderabad, Telangana, INDIA.
Keywords: E. acaulis L, Anticancer activity, Cytotoxic assay, Propidium iodide, Apoptosis

Abstract

Objectives: In the present study, the anticancer potential of the plant extracts of Elytraria acaulis L. (E. acaulis L.) were investigated on human breast cancer cells (MDAMB-231). Methods: Different organic solvent extras.cts of E. acaulis L. were prepared using methanol, ethanol, ethyl acetate and hexane. The cytotoxicity of plant extracts were evaluated by MTT assay (-(4, 5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide) against MDA MB-231cells. The apoptosis associated morphological changes were also observed with the help of propidium iodide staining method. Results: Of all the solvent extracts, methanol extract has shown the highest activity and its IC50 concentration was found to be 80.8±2.08 μg/ml. The anticancer drug Cis-diammineplatinum (II) dichloride was considered as a standard. Conclusion: In further the bioactive compound should be isolated from the methanol crude extract, which is responsible for the cytotoxic effect

Downloads

Download data is not yet available.
Graphical illustration showing cytotoxic effect of different concentrations of cisplatin on MDA MB 231 after 24 hr treatment.
Published
2021-12-28
How to Cite
1.
Bastipati SB, Kalyani C, Tulasi C, Saida L. Anticancer Activity of Elytraria acaulis L. Extracts on Triple Negative Breast Cancer Cell Line. ijpi [Internet]. 28Dec.2021 [cited 4Oct.2022];11(4):354-7. Available from: https://jpionline.org/index.php/ijpi/article/view/1165